Blood collection

Blood collection


LASA850: Response blood sampling / DNA
LASA851: Date blood sampling (including time point when available)
LASA860: Routine measurements

Contact: Natasja van Schoor

Blood collection first cohort (B-, C- and G-cycle)
At the LASA B-cycle (1992/93), blood was collected in the regions Amsterdam and Zwolle; no blood was collected in Oss. Non-fasting blood samples were taken during the medical interview while the subject was sitting. The blood that was obtained during this cycle was centrifuged and stored at -80° until determination. Several routine determinations were performed immediately, this concerns blood of the region Zwolle and part of the region Amsterdam (Table 5). In the region Zwolle this was done in the ISALA clinic; in Amsterdam this was done in the PCA Valeriuskliniek. See Table 1 for the response rates.

At the LASA C-cycle (1995/96), blood samples were obtained from respondents who participated in the medical interview of LASA (1995/96), were born in 1930 and before (aged 65 years and older as of 1 January, 1996) and were living in Amsterdam, Zwolle and Oss and surroundings. Of the 1,720 respondents who were eligible for the medical interview, 1,509 took part in the medical interview. After this interview at home, participants were invited to the VUmc (respondents living in Amsterdam and vicinity) or a health care center near their homes (respondents living in Zwolle or Oss and vicinity), where blood samples were obtained. For respondents who were not able to come to the VUmc, blood samples were drawn at home (n=76). Blood samples were available for 1,331 respondents. At the visit in the hospital or health care center, fasting urine and blood samples were obtained, centrifuged and stored at -80° or analyzed immediately. In the region Zwolle the immediate assays were done in the ISALA clinic; in Amsterdam this was done in the VUmc; in Oss, this was done in the Bernhoven Hospital. Respondents were allowed to take tea and toast, but no dairy products. In Amsterdam, morning blood samples were collected (between 8.30 and 9.30 a.m.); in Zwolle, blood samples were collected during the daytime; in Oss, morning blood samples were collected (between 8.00 and 10.00 a.m.).. See Table 1 for the response rates.

At the LASA G-cycle (2008/09), fasting blood samples were drawn in Amsterdam, Zwolle and Oss and surroundings. Subjects were not allowed to take any food or drinks from midnight. Of 58 respondents, blood samples were drawn at home. The other participants were sampled on location. The samples were centrifuged and stored at -80° or analyzed immediately. See Table 1 for the response rates.

In general:
Blood sampling took place within 4 to 6 weeks after the medical interview of LASA, except for the B-cycle where blood sampling took place at home during the medical interview. Routine determinations were performed immediately after blood sampling in the lab where the blood was collected (in case of using health care centers near the home of a respondent, or home visit, the blood was transported to the central laboratory of each region). Samples were centrifuged within 4 hours. It should be noted that different labs were used in the different regions. Furthermore, when comparing routine measurements performed in fresh blood over time, it should be noted that at the B-cycle a different laboratory was used in Amsterdam (PCA Valeriuskliniek) than at later cycles (VUmc) . All blood samples are stored at -80°. However, in case of a new determination in blood, the blood is temporarily stored at the laboratory at -20°.

Laboratory determination
Albumin, creatinine and total cholesterol were determined as routine measurements in fresh blood at LASA B and C; fasting glucose was additionally assessed at LASA G. For an overview of the most important biomaterial measurements, see Table 4. For a detailed overview of routine measurements, see Table 5.

Table 1. Response first cohort

Cycle

Total medical interview

Number of blood samples

Response (%)

B1

2675 (2671)2

17843

66.7

C

1509

1331

88.2

G4

761

400

52.6

1 No blood was collected in Oss in the B-cycle.
2 In 4 persons, data of the medical interview were lost.
3 In part of Amsterdam blood was collected but routine measurements were not determined immediately (n=502) in the B-cycle.
4 At the G-cycle, a combined blood collection was performed in the first and second cohort. Reported numbers include only the first cohort.

Blood collection second cohort (2B- and G-cycle)
At the LASA 2B cycle (2002/03), blood samples were collected in Amsterdam, Zwolle and Oss and surroundings. Respondents were allowed to take tea and toast, but no dairy products. In Amsterdam, morning blood samples were collected (between 8.30 and 9.30 a.m.); in Zwolle, blood samples were collected during daytime; in Oss, morning blood samples were collected (between 8.00 and 10.00 a.m.). Of 15 participants, blood samples were drawn at home. The other participants were sampled on location. The samples were centrifuged and stored at -80° or analyzed immediately. See Table 2 for the response rates.

At the LASA G-cycle (2008/09), fasting blood samples were drawn in Amsterdam, Zwolle Oss and surroundings. Subjects were not allowed to take any food or drinks from midnight. Of 11 participants, blood samples were drawn at home. The other participants were sampled on location. The samples were centrifuged and stored at -80° or analyzed immediately. See Table 2 for the response rates.

Laboratory determination
Albumin, creatinine and total cholesterol were determined as routine measurements in fresh blood at LASA 2B; fasting glucose was additionally assessed at LASA G. For an overview of the most important biomaterial measurements, see Table 4. For a detailed overview of routine measurements, see Table 6.

Table 2. Response second cohort

Cycle

Total medical interview

Number of blood samples

Response (%)

2B

919

748

81.4

G1

733

535

73.1

1 At the G-cycle, a combined blood collection was performed in the first and second cohort. Reported numbers include only the second cohort.

Blood collection third cohort (3B cycle)
At the 3B-cycle (2012/13), fasting blood samples were drawn. Subjects were not allowed to take any food or drinks from midnight. The samples were centrifuged and stored at -80° or analyzed immediately. See Table 3, for the response rates.

Laboratory determination
Fasting glucose was assessed as a routine measurement in fresh blood (Table 4).

Table 3. Response third cohort

Cycle

Total medical interview

Number of blood samples

Response (%)

3B

889

TBD

TBD

TBD=To be determined


Table 4. Most important biomaterial measurements and realized sample size (published in the European Journal of Epidemiology 2016) 

Biomaterial

Availability in LASA (n)

Routine measurements in fresh blood

B

C

2B

G

3B

Albumin

1507

1326

747

924

 

Creatinine

1507

1327

747

925

-

Total cholesterol

1507

1309

744

923

-

Fasting glucose

-

-

-

918

640

 

Measurements in frozen blood a

B

C

2B

G

3B

Total cholesterol 

-

1296

-

-

NYA b

High-density lipoprotein (HDL) cholesterol

-

1295

-

-

Low-density lipoprotein (LDL) cholesterol

-

1287

-

-

Triglycerides

-

1296

-

-

Fructosamine

-

1298

-

-

Adipokines (adiponectin, leptin, ghrelin)

-

-

-

897

Total plasma cortisol

-

1279

-

-

Cortisol binding globulin (CBG)

-

1277

-

-

Homocysteine

-

1301

-

-

Vitamin B12

-

1276

-

-

Methylmalonic acid (MMA)

-

582

-

-

Sex hormone-binding globulin (SHBG) 

-

1320

-

-

Insulin-like growth factor 1 (IGF-1)

-

1319

-

-

Osteocalcin (OC)

-

1319

-

-

Pro-inflammatory cytokine Interleukin-6 (IL-6)

1745

1287

-

-

Acute-phase proteins C-reactive protein (CRP)

1744

1287

-

-

Alpha-1-antichymotrypsin (ACT)

1743

1284

-

-

Serum 25-hydroxyvitamin D (25-OHD)

-

1320

739

915

Parathyroid hormone (PTH)

-

1320

739

915

Follicle stimulating hormone (FSH)

-

1301

-

-

Luteinizing hormone (LH)

-

1301

-

-

Testosterone

-

630

-

-

Estradiol

-

1301

-

-

Thyroid stimulating hormone (TSH) c

-

1270

-

-

Vitamin K (dp-ucMGP + dp-cMGP)

-

-

696

-

 

Other measurements

C

E

Ancillary study (2010-11)

Urine deoxypyridinolin (DPD)

1307

-

-

Urine creatinine

1311

-

-

Salivary cortisol

-

1199

-

Hair cortisol

-

-

333

 

DNA

First cohort

Second cohort

Third cohort

Exome chip analysis

1193

-

NYAa

Apolipoprotein E (ApoE) phenotyping

2233

-

Apolipoprotein E (ApoE) genotyping

-

753

Collagen type I α1 Sp1 polymorphism

966

-

Single nucleotide polymorphisms Vitamin D Receptor

935

-

Single nucleotide polymorphisms Glucocorticoid Receptor

935

-

aNew blood determinations may become available in the future from frozen blood.
bNot yet available: Blood has been collected (n = 640), but assessments have not yet been performed.
cIn case of abnormalities in TSH level, Tri-jodothyronin (T3) and/or Thyroxin (T4) were measured.


Table 5. Detailed overview of routine measurements in fresh blood in first and second cohorta

 

First cohort

Second cohort

B-cycle (n=2675)

C-cycle (n=1509)

G-cycle (n=761)

2B-cycle(n=919)

G-cycle (n=733)

 

N= valid data

N= valid data

N= valid data

N= valid data

N= valid data

Haemoglobine mMol/L

1509

400

 

282

 

Haematocriet LL

1508

400

 

280

 

Erythrocyten x(10 power 12)/L

1508

400

 

280

 

Leucocyten x(10 power 9)/L

1494

398

 

280

 

Multi cellulair hamoglobine a Mol

736

 

 

 

 

Multi cellulair volume FL

736

 

 

 

 

Multi cellulair haemoglobine concentration mMol/L

736

 

 

 

 

Creatinine uMol/L

1507

1327

395

747

530

Albumine g/L

1507

1326

395

747

529

Total cholesterol mMol/L

1507

1309

395

744

528

Cholesterol/HDL ratio

1502

404

 

283

 

Ferro uMol/L

773

404

 

283

 

Vitamine B12 pMol/L

767

404

 

283

 

Foliumacid nMol/L

772

400

 

283

 

TSH mU/L

769

398

 

283

 

CRP mG/L

223

404

 

283

 

Calcium mMol/L

773

404

 

283

 

Ureum mMol/L

773

404

 

283

 

Total Protein G/L

773

404

 

283

 

OT (ASAT) U/L

773

404

 

283

 

PT (ASAT) U/L

773

404

 

283

 

Gamma GT U/L

773

404

 

283

 

Glucose mMol/L

773

404

391

283

527

Triglyceriden mMol/L

773

404

 

283

 

HDL mMol/L

770

404

 

283

 

Free T4 pMol/L

773

404

 

283

 

LDL mmol/L

768

404

 

283

 

BSE mm/U

1505

398

 

276

 

a Not necessarily performed in same selection of participants.